\u8eab\u4f53\u306e\u7d30\u80de\u306e\u7a2e\u985e\u3092\u4e00\u3064\u4e00\u3064\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u6700\u3082\u78ba\u304b\u306a\u65b9\u6cd5\u306f\u3001\u305d\u306e\u7d30\u80de\u306eDNA\u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u3092\u77e5\u308b\u3053\u3068\u3067\u3059(single cell transcriptomics)\u3002\u4e00\u4eba\u306e\u7d30\u80de\u306eDNA\u306f\u5168\u8eab\u3067\u540c\u3058\u3067\u3082\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u3069\u306e\u907a\u4f1d\u5b50\u304c\u767a\u73fe\u3057\u3066\u5fc5\u8981\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f5c\u3063\u3066\u3044\u308b\u304b\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u6790(proteomics)\u306f\u5927\u5909\u3067\u3059\u304c\u3001\u305d\u306e\u624b\u524d\u306emRNA\u306fPCR\u6cd5\u3092\u4f7f\u3063\u3066\u5fae\u91cf\u3067\u3082\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002
\nhttps:\/\/en.wikipedia.org\/wiki\/Single-cell_transcriptomics
\n\u306b\u624b\u6cd5\u304c\u89e3\u8aac\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u3061\u3087\u3063\u3068\u7c21\u7565\uff09\u3002
\nhttps:\/\/www.nature.com\/articles\/nn.4366\u3000\u3082\u5177\u4f53\u7684\u306a\u624b\u6cd5\u304c\u308f\u304b\u308a\u3084\u3059\u3044\u3067\u3059\u3002
\n\u30fb\u7d30\u80de\u4e00\u3064\u3092\u5206\u96e2\u3059\u308b\u306b\u306f\u3001\u30d1\u30d1\u30a4\u30f3\u306a\u3069\u306e\u6d88\u5316\u9175\u7d20\u3067\u7d30\u80de\u306e\u63a5\u7740\u90e8\u5206\u3092\u6eb6\u304b\u3057\u3066\u30d0\u30e9\u30d0\u30e9\u306b\u3057\u3066\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3092\u4f7f\u3046\u3001\u7d30\u80de\u8cea\u5438\u5f15\uff08\uff1f cytoplasmic aspiration\uff09\u3001\u30ec\u30fc\u30b6\u30fc\u306e\u5149\u30d4\u30f3\u30bb\u30c3\u30c8\u3067\u6355\u307e\u3048\u3066\u5206\u96e2\u3059\u308b\u3001\u6db2\u6ef4\uff11\u3064\u306b\u7d30\u80de1\u500b\u304c\u5165\u308b\u3088\u3046\u306b\u3057\u3066\u6db2\u6ef4\u3092\u3068\u3070\u3057\u3001\u86cd\u5149\u3092\u767a\u3059\u308b\u7d30\u80de\u304c\u5165\u3063\u305f\u3082\u306e\u3060\u3051\u3092\u96fb\u5834\u3067\u3088\u308a\u5206\u3051\u308b\uff08FACS\uff09\u306a\u3069\u3044\u308d\u3044\u308d\u306a\u624b\u6cd5\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u6700\u8fd1\u306f\u30de\u30a4\u30af\u30ed\u6d41\u8def\u304c\u4f7f\u308f\u308c\u308b\u305d\u3046\u3067\u3059\u3002
\n\u30fb\u5b9a\u91cf\u7684PCR\u3000\u6e2c\u308a\u305f\u3044\u907a\u4f1d\u5b50\u306b\u5bfe\u5fdc\u3059\u308bprimer\u914d\u5217\u3092\u4f7f\u3063\u3066PCR\u5897\u5e45\u3057\u3001\u5fc5\u305a\u5b58\u5728\u3059\u308b\u907a\u4f1d\u5b50\uff08housekeeping genes\uff09\u306e\u5f37\u5ea6\u3067\u898f\u683c\u5316\u3057\u307e\u3059\u3002\u5897\u5e45\u5f8c\u306e\u691c\u51fa\u306f\u76f8\u88dc\u7684\u306aRNA\u306b\u86cd\u5149\u8272\u7d20\u3092\u3064\u3051\u3066\u86cd\u5149\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u305d\u3046\u3067\u3059\u3002nm\u306e\u5f84\u306e\u5b54\u3092RNA\u304c\u901a\u308b\u6642\u306e\u96fb\u6c17\u62b5\u6297\u5909\u5316\u3092\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3082\u3042\u308a\u3001\u691c\u7d22\u3059\u308b\u3068\u4e00\u756a\u4e0a\u306b\u305d\u306e\u6a5f\u5668\u3092\u4f5c\u3063\u3066\u3044\u308b\u4f1a\u793e\u306e\u30da\u30fc\u30b8\u304c\u51fa\u3066\u304d\u307e\u3059\u3002
\n\u30fb\u5358\u4e00\u7d30\u80deRNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3000\u3053\u308c\u306f\u4e0a\u8a18\u3068\u306f\u9055\u3046\u65b9\u6cd5\u306e\u3088\u3046\u3067\u3001mRNA\u304b\u3089\u76f8\u88dc\u7684DNA(cDNA)\u65ad\u7247\u3092\u4f5c\u308a\u3001\u305d\u306e\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3068\u30c7\u30fc\u30bf\u51e6\u7406\u3067mRNA\u306e\u767a\u73fe\u72b6\u614b\u3092\u8abf\u3079\u308b\u3088\u3046\u3067\u3059\u3002
\n\u51fa\u3066\u304d\u305f\u30c7\u30fc\u30bf\u306f\u3001\u6a5f\u68b0\u5b66\u7fd2\u306e\u624b\u6cd5\u3067\u5206\u985e\u3055\u308c\u307e\u3059\u3002\u30af\u30e9\u30b9\u30bf\u30ea\u30f3\u30b0\u3001\u6b21\u5143\u524a\u6e1b\u306a\u3069\u304a\u306a\u3058\u307f\u306e\u624b\u6cd5\u306b\u52a0\u3048\u3066\u3001Differential expression, gene ontology enrichment, pseudotempral ordering, trajectory inference \u306a\u3069\u3001\u3053\u306e\u696d\u754c\u306b\u72ec\u7279\u306e\u624b\u6cd5\u3082\u89e3\u8aac\u3055\u308c\u3066\u3044\u307e\u3059\u3002ontology \uff08\u30aa\u30f3\u30c8\u30ed\u30b8\u30fc\uff09\u3068\u3044\u3046\u306e\u306f\u8272\u3005\u306a\u6982\u5ff5\u304c\u3064\u306a\u304c\u3063\u305f\u69cb\u9020\u3092\u56f3\u793a\u3057\u305f\u3082\u306e\u3067\u3001\u751f\u7269\u60c5\u5831\u306e\u8ad6\u6587\u3067\u3088\u304f\u51fa\u3066\u304d\u307e\u3059\u3002\u3082\u3068\u3082\u3068\u306f\u54f2\u5b66\u306e\u300c\u5b58\u5728\u8ad6\u300d\u306e\u610f\u5473\u3067\u3057\u305f\u304c\u3001\u6700\u8fd1\u306f\u751f\u7269\u5b66\u7684\u73fe\u8c61\u2192\u5206\u5b50\u30ec\u30d9\u30eb\u306e\u610f\u5473\u4ed8\u3051\u306e\u6587\u8108\u3067\u898b\u307e\u3059\u3002\u300cG\u691c\u5b9a\u300d\u3067\u306f\u3001\u4eba\u9593\u306e\u6301\u3064\u60c5\u5831\u3092\u30b3\u30f3\u30d4\u30e5\u30fc\u30bf\u304c\u308f\u304b\u308b\u3088\u3046\u306b\u6574\u7406\u8a18\u8ff0\u3059\u308b\u3053\u3068\u3001\u307e\u305f\u305d\u306e\u30d5\u30ec\u30fc\u30e0\u30ef\u30fc\u30af\u3092\u6307\u3059\u3001\u3068\u4e0b\u8a18\u306e\u89e3\u8aac\u306b\u3042\u308a\u307e\u3059\u3002\u3061\u3087\u3063\u3068\u3075\u308f\u3075\u308f\u3057\u305f\u8a00\u8449\u3067\u3001\u76f8\u624b\u3092\u3051\u3080\u306b\u5dfb\u304f\u3068\u304d\u306b\u4f7f\u308f\u308c\u308b\u304b\u3082\u3057\u308c\u306a\u3044\u306e\u3067\u3001\u6587\u8108\u306b\u8981\u6ce8\u610f\u3067\u3059\u3002
\nhttps:\/\/zero2one.jp\/ai-word\/ontology\/<\/p>\n
\u82f1\u8a9e\u306f\u3000https:\/\/en.wikipedia.org\/wiki\/Single-cell_transcriptomics\u3000\u305d\u306e\u4ed6\u304b\u3089\u3002
\n-omics \uff5e\u5b66\u30fb\u5206\u91ce\uff08\u751f\u7269\u5b66\u3067\u4f7f\u3046\uff09\u3000\u3000proteomics \u30bf\u30f3\u30d1\u30af\u8cea\u5b66\u3000\u3000metabolomics \u4ee3\u8b1d\u7269\u8cea\u5b66 \u3000transcriptomics\u3000\u8ee2\u5199\u7523\u7269\uff08mRNA\uff09\u5b66\u3000\u7121\u7406\u306b\u8a33\u3055\u306a\u3044\u3067\u30ab\u30bf\u30ab\u30ca\u8a9e\u3068\u3057\u3066\u4f7f\u308f\u308c\u307e\u3059\u3002
\nontology \u30aa\u30f3\u30c8\u30ed\u30b8\u30fc
\noncology \u816b\u760d\u5b66
\ncytoplasm \u539f\u5f62\u8cea
\ninference \u63a8\u5b9a \u300c\u30a4\u300d\u30f3\u30d5\u30a7\u30ec\u30f3\u30b9\u3000Bayes inference \u30d9\u30a4\u30ba\u63a8\u5b9a
\n“This trajectory of cells can be linear, but can also bifurcate or follow more complex graph structures. The trajectory, therefore, enables the inference of gene expression dynamics and the ordering of cells by their progression through differentiation or response to external stimuli.”
\n\u3053\u306e\u7d30\u80de\u306e\u8ecc\u8de1\u306f\u7dda\u72b6\u3067\u3082\u3088\u3044\u304c\u3001\u5206\u5c90\u3057\u305f\u308a\u3082\u3063\u3068\u8907\u96d1\u306a\u30b0\u30e9\u30d5\u69cb\u9020\u306b\u5f93\u3063\u305f\u308a\u3057\u3066\u3082\u3088\u3044\u3002\u306a\u306e\u3067\u3001\u3053\u306e\u8ecc\u8de1\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u30c0\u30a4\u30ca\u30df\u30af\u30b9\u3084\u5206\u5316\u306e\u904e\u7a0b\u3067\u306e\u5f62\u614b\u5909\u5316\u306b\u3088\u308b\u7d30\u80de\u306e\u5e8f\u5217\u5316\u3084\u5916\u90e8\u523a\u6fc0\u306b\u5bfe\u3059\u308b\u5fdc\u7b54\u3092\u63a8\u5b9a\u3059\u308b\u306e\u306b\u4f7f\u3048\u308b\u3002
\ntrajectory \u8ecc\u8de1
\nbifurcation \u30d0\u30a4\u30d5\u30a1\u30b1\u2015\u30b7\u30e7\u30f3\u3000\u5206\u5c90
\ndifferentiation \u5206\u5316
\nstimuli \u30b9\u30c6\u30a3\u30df\u30e5\u3089\u30a4\u3000\u523a\u6fc0<\/p>\n","protected":false},"excerpt":{"rendered":"
\u8eab\u4f53\u306e\u7d30\u80de\u306e\u7a2e\u985e\u3092\u4e00\u3064\u4e00\u3064\u540c\u5b9a\u3059\u308b\u305f\u3081\u306e\u6700\u3082\u78ba\u304b\u306a\u65b9\u6cd5\u306f\u3001\u305d\u306e\u7d30\u80de\u306eDNA\u767a\u73fe\u30d1\u30bf\u30fc\u30f3\u3092\u77e5\u308b\u3053\u3068\u3067\u3059(single cell transcriptomics)\u3002\u4e00\u4eba\u306e\u7d30\u80de\u306eDNA\u306f\u5168\u8eab\u3067\u540c\u3058\u3067\u3082\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u3069\u306e\u907a\u4f1d\u5b50\u304c\u767a\u73fe\u3057\u3066\u5fc5\u8981\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4f5c\u3063\u3066\u3044\u308b\u304b\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u6790(proteomics)\u306f\u5927\u5909\u3067\u3059\u304c\u3001\u305d\u306e\u624b\u524d\u306emRNA\u306fPCR\u6cd5\u3092\u4f7f\u3063\u3066\u5fae\u91cf\u3067\u3082\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 https:\/\/en.wikipedia.org\/wiki\/Single-cell_transcriptomics \u306b\u624b\u6cd5\u304c\u89e3\u8aac\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u3061\u3087\u3063\u3068\u7c21\u7565\uff09\u3002 https:\/\/www.nature.c…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[42,46],"tags":[5,9],"class_list":["post-2088","post","type-post","status-publish","format-standard","hentry","category-tech","category-bio","tag-tech","tag-bio"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=\/wp\/v2\/posts\/2088","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2088"}],"version-history":[{"count":0,"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=\/wp\/v2\/posts\/2088\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2088"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2088"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sekaiken.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2088"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}